Cannabis plant named ‘PG 1 19 0125 0002’

ABSTRACT

A new and distinct  Cannabis  cultivar named ‘PG 1 19 0125 0002’ is characterized by its dominant CBG chemotype, which is favorable for application as a medicinal product. Furthermore, it may be used for the industrial-scale extraction of CBG due to the minimal concentrations of contaminating cannabinoids which may otherwise complicate the extraction of this class of secondary metabolites.

Latin name of the genus claimed: Cannabis Hybrid.

Variety denomination: ‘PG 1 19 0125 0002’.

BACKGROUND AND SUMMARY OF THE INVENTION

A novel Cannabis hybrid cultivar, entitled ‘PG 1 19 0125 0002’ isprovided. ‘PG 1 19 0125 0002’ is the result of a planned breedingprogram and originated from crosses between privately-owned cultivars.The new cultivar has been vegetatively reproduced by cloning using stemcuttings at Zeiningen, Switzerland. Vegetative clones of ‘PG 1 19 01250002’ were tested in controlled indoor growth facilities, greenhouses,and outdoors in open fields. The desired characteristics of each sourcecultivar are transferred by vegetative, asexual reproduction. ‘PG 1 190125 0002’ is stable and consistently true-to-type through multiplegenerations of vegetative reproduction.

Cannabis is a genus of flowering plants comprising three historicallydistinct subspecies based on phenotype and metabolite profiles—Cannabissativa, Cannabis indica, and Cannabis ruderalis. However, decades ofcrossing and selection makes it impossible to absolutely characterizethe resulting hybrid plants using phenotypic data. Most of the Cannabisvarieties being sold for medicinal and recreational purposes containscharacteristics of both Cannabis sativa and Cannabis indica subspecies.For this reason, ‘PG 1 19 0125 0002’, described herein, has beencharacterized both on the presented phenotype, as well as the genotypeusing a series of single nucleotide polymorphisms (SNP).

Used herein, the terms “cultivar”, “variety”, “clone” and “strain” areused interchangeably.

Cannabis plants synthesize unique terpeno-phenolic compounds in varyingconcentrations. High genetic variability has resulted in the vastvarieties of chemotypes with distinct characteristics available today.More than 500 unique compounds including cannabinoids, terpenoids,terpenes, flavonoids, amino acids, vitamins among many others, aresecreted as a sticky resin by the glandular trichomes found on thefloral calyxes of female plants. Cannabinoids and terpenes are thebiologically active chemicals responsible for the pharmacological andpsychoactive properties of Cannabis when consumed by humans. They oftenwork together synergistically in what is commonly known as the“entourage effect” and as such, small differences in composition orconcentration any of these compounds can have notable effects on thephysiological effect of consumed or applied Cannabis in or on the humanbody.

Cannabinoids are produced at significant concentrations in Cannabis.Although over a hundred cannabinoids have been identified in Cannabis,the major cannabinoids include, Δ9-tetrahydrocannabinol (THC),cannabidiol (CBD), cannabigerol (CBG), cannabinol (CBN), cannabichromene(CBC), cannabinodiol (CBDL), cannabicyclol (CBL), cannabivarin (CBDV),cannabigerovarin (CBGV), cannabichromevarin (CBCV),tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabigerolmonomethyl ether (CBGM), cannabilsoin (CBE), cannabicitran (CBT),cannabinol propyl variant (CBNV), cannabitriol (CBO),tetrahydrocannabinolic acid (THCA), tetrahydrocannabivarinic acid(THCVA), cannabidiolic acid (CBDA), cannabigerolic acid (CBGA) andcannabinerolic acid. These cannabinoids are usually produced by theplant in their acid form (see suffix -A), but upon heating they become.decarboxylated.

CBGA is the precursor to CBDA and THCA. CBG is usually present at verylow concentrations due to its rapid utilization by CBDA or THCAsynthases. It is non-psychoactive and has been described to displayanalgesic, antibiotic and anti-inflammatory effects. It has further beenshown to reduce blood clotting and to relieve the intraocular pressureexperienced by glaucoma patients. CBG further reduces the effect of THCand is actively being studied in its acid and neutral forms in manymedical conditions.

As used herein, CBG will refer to both the decarboxylated form, CBG, andCBGA. The value “Total CBG” will refer to the calculated sum of bothisoforms when decarboxylated i.e. Total CBG=GBGA×87.8%+CBG. Total THCand CBD are similarly calculated but with a conversion factor of 87.7%.

Terpenes are organic molecules produced by various plants and animals.These organic compounds are responsible for the strong and distinctivesmell and taste of Cannabis. Some of the most prominent terpenes foundin Cannabis are myrcene, caryophyllene, and limonene, to mention a few.

The objective of the breeding program that produced ‘PG 1 19 0125 0002’was to develop Cannabis plant varieties with unique combinations ofcannabinoids and/or terpenes, while displaying a phenotype with multiplelarge inflorescences arranged in a naturally branched habit. The varietydescribed herein is a result of this breeding program.

The ‘PG 1 19 0125 0002’ cultivar was the result of poly-cross betweenseveral hemp-type and high CBD-type Cannabis plants. Selected progenieswere then cloned and crossed by selfing using methods known in the art.In subsequent generations, the resultant plants were screened for hightotal CBG production and selected based on production-relevantphenotypes. The progeny with the most stable desirable chemotype wasassigned the name, ‘PG 1 19 0125 0002’.

The ‘PG 1 19 0125 0002’ plants described herein were grown in Zeiningen,Switzerland, in nurseries, climate-controlled growth facilities, and inthe field during the summer of 2019. Samples for metabolite analysiswere taken from the flowers of numerous plants. Analytical measurementswere made using Ultra Performance Liquid Chromatography by those skilledin the art. Plant phenotyping was performed using photography andsubsequent analysis.

The ‘PG 1 19 0125 0002’ variety described herein is extremely low incontaminating cannabinoids such as THC and CBD allowing for easierextraction of the CBG contained in the flowers. In addition, the low THCconcentration facilitates extraction in that cannabinoids can beconcentrated to a higher level, during the extraction process.

‘PG 1 19 0125 0002’ flowers were analyzed at harvest in both indoorgrowth and outdoor growth environments (Table 1). The resultantcannabinoid profile highlights the uniquely high CBG, and low THC andCBD content characterizing the ‘PG 1 19 0125 0002’ variety. It has acannabinoid profile in the dried mature female flowers that is dominantin CBG (>5% w/w) and displays only trace amounts of THC (<0.1% w/w) andCBD (<0.1% w/w).

‘PG 1 19 0125 0002’ presents with a low total terpene content. In freshflowers the total measurable terpene content is between 0.05 and 0.07%(w/w) in the fresh flowers, and between 0.9 and 0.12% (w/w) in driedflowers. The dominant terpene found in fresh flowers is Myrcene followedby trans-Caryophyllene and Limonene (Table 2). The unique profile of themajor and minor terpenes makes ‘PG 1 19 0125 0002’ distinguishable fromother varieties, including Santhica 27 and PG_1_Pre-19_0125_0004.

TABLE 1 Cannabinoid Concentrations in ‘PG 1 19 0125 0002’ PG_1_Pre- ‘PG1 19 0125 0002’ Santhica 27 19_0125_0004 Indoor Outdoor Outdoor OutdoorFlowers Flowers Flowers Flowers Range of active cannabinoids (% byweight) TOTAL CBG  7.2-8.105   5.6-9.442 0.4-1.4 11.5-15.5 TOTAL THC0.07-0.13 0.053-0.13 — 0.45-0.75 TOTAL CBD   0-0.03 0.001-0.21   0-0.20.85-1.34 CBC 0.03-0.29  0.03-0.39 —   0-0.15

TABLE 2 Terpene Concentrations in ‘PG 1 19 0125 0002’ Fresh Flowers DryFlowers Ranges of Terpenes (% by weight) alpha-Pinene 0.000-0.001% —(−)-beta-Pinene 0.001-0.001% — Myrcene 0.028-0.031% 0.010-0.012%(R)-(+)-Limonene 0.004-0.006% 0.001-0.002% p-Cymene 0.00% 0.005-0.008%Linalool 0.001-0.003% 0.00% L-Fenchone — 0.003-0.006% (+)-Fenchol 0.00%— (−)-Isopulegol 0.000-0.001% — (−)-alpha-Terpineol 0.00% — CitralIsomer 2 0.000-0.001% 0.002-0.003% (−)-trans-Caryophyllene 0.007-0.009%0.003-0.009% alpha-Humulene 0.002-0.003% 0.002-0.003% Nerolidol Isomer 20.001-0.004% 0.004-0.009% (−)-alpha-Bisabolol 0.003-0.004% 0.009-0.016%Phytol Isomer 2 0.003-0.006% 0.037-0.060%

The genotype of ‘PG 1 19 0125 0002’ was characterized usingGenotyping-by-sequencing (GBS) using methods described in the art.Short-read sequencing data produced by this method were aligned to thepublicly available assembled genome of cs10 (Assembly: GCF_900626175.1)to identify single nucleotide polymorphisms (SNP) and short haplotypes(HAP, a combination of linked SNPs) in ‘PG 1 19 0125 0002’ (Table 3 andFIG. 14).

Together these polymorphisms provide a fingerprint which can be used todifferentiate ‘PG 1 19 0125 0002’ from all other Cannabis varieties.

Table 3 shows a list of single nucleotide polymorphisms (SNPs) andhaplotypes (HAPs) contained within the genome of ‘PG 1 19 0125 0002’compared to the publicly available reference genome of cs10 (Assembly:GCF_900626175.1). Each record shows the reference chromosome of cs10,the nucleotide position on the cs10 reference sequence, the nucleotidesequence at this position, the nucleotide sequence displayed in ‘PG 1 190125 0002’, and whether the state of the polymorphism is homozygous orheterozygous in ‘PG 1 19 0125 0002’. Heterozygous loci contain oneallele sequence identical to the reference nucleotide sequence and oneallele of the ‘PG 1 19 0125 0002’ sequence.

TABLE 3 Reference Position in Reference ‘PG 1 19 chromosome reference nucleotide 0125 0002’ (cs10) sequence  sequence Sequence StateNC_044370.1 2085232 AA AT homozygous NC_044370.1 4838286 CGT CGChomozygous NC_044370.1 36467493 C T homozygous NC_044370.1 11271773 CACG homozygous NC_044370.1 11271954 CAG AAG homozygous NC_044370.113526608 CAA CTG homozygous NC_044379.1 57862779 C T homozygousNC_044379.1 57863241 GGTAC AAAGC homozygous NC_044379.1 57915145 TT TChomozygous NC_044379.1 57954931 C G homozygous NC_044379.1 58169058 GTGCAA homozygous NC_044379.1 58292575 ATCCAA GTCCCA homozygous NC_044379.158292927 T C homozygous NC_044379.1 26744250 G T homozygous NC_044379.161946066 A G homozygous NC_044379.1 62821655 G C homozygous NC_044371.13483276 ACG ACA homozygous NC_044371.1 66149411 T A homozygousNC_044371.1 75422669 TGA CGT homozygous NC_044371.1 75974152 GCC GTChomozygous NC_044371.1 81582801 TT CT homozygous NC_044371.1 82218848GAG AGT homozygous NC_044371.1 82483522 GC AA homozygous NC_044371.130915413 A G homozygous NC_044372.1 2710529 T C homozygous NC_044372.12710755 TG CG homozygous NC_044372.1 80252454 G T homozygous NC_044372.11890501 T C homozygous NC_044373.1 7589246 CA GA homozygous NC_044373.116326218 AA GG homozygous NC_044373.1 18857773 T G homozygousNC_044373.1 26968201 TCC TCT homozygous NC_044373.1 29630917 G Ahomozygous NC_044373.1 44999598 C T homozygous NC_044373.1 46220665 CTTG homozygous NC_044373.1 65997446 C T homozygous NC_044373.1 65997630 AG homozygous NC_044373.1 78468284 G C homozygous NC_044373.1 90475592AAGG AAAA homozygous NC_044373.1 91302456 ATAA CTAA homozygousNC_044374.1 721191 GC CC homozygous NC_044374.1 2380328 T A homozygousNC_044374.1 7842585 C T homozygous NC_044374.1 46733863 T A homozygousNC_044374.1 81289150 C T homozygous NC_044374.1 87026417 CT GThomozygous NC_044374.1 53042056 C G homozygous NC_044374.1 75148552 C Thomozygous NC_044374.1 79606305 GCG CTA homozygous NC_044374.1 40728787A G homozygous NC_044374.1 81665769 ATGC ATGT homozygous NC_044375.193189088 A G homozygous NC_044375.1 5028137 CGT TGT homozygousNC_044375.1 5229457 TAT CAT homozygous NC_044375.1 92418831 AGCC AACChomozygous NC_044375.1 36259780 GA GC homozygous NC_044375.1 27969778 GAGG homozygous NC_044375.1 47220146 C G homozygous NC_044376.1 3765575CCT TTT homozygous NC_044376.1 4038278 AGG CAG homozygous NC_044376.135656963 TGG CGA homozygous NC_044376.1 41113525 GACG GTCG homozygousNC_044376.1 49690671 A G homozygous NC_044376.1 57526518 TC GChomozygous NC_044376.1 61093783 TA AG homozygous NC_044377.1 491424 CTGCAT homozygous NC_044377.1 570205 TCT AAT homozygous NC_044377.122400517 A G homozygous NC_044377.1 29225173 T A homozygous NC_044377.136155194 AGG CGG homozygous NC_044377.1 44377730 GG CG homozygousNC_044377.1 53006255 A T homozygous NC_044377.1 61322716 AGC AAAhomozygous NC_044377.1 72446888 G A homozygous NC_044377.1 78287396TTGTC GTGTC homozygous NC_044378.1 108801 TT TC homozygous NC_044378.1459898 TATA TTCA homozygous NC_044378.1 19944962 GC AG homozygousNC_044378.1 23972788 CT CA homozygous NC_044378.1 29436763 CCCCCC ACCCTThomozygous NC_044378.1 46687062 GGCG GGCA homozygous NC_044378.153550861 CAGG TGGA homozygous NC_044378.1 63538044 GT GA homozygousNC_044378.1 66753962 GGGT GAGT homozygous NC_044370.1 72116963 CC TTheterozygous NC_044370.1 104558715 AC CC heterozygous NC_044371.142597265 TT TC heterozygous NC_044372.1 35317415 CTC GTC heterozygousNC_044372.1 63982910 GGCG GCCG heterozygous NC_044375.1 69785254 GCG TTAheterozygous NC_044379.1 12989226 CT TC heterozygous NC_044379.141567327 CAAAC ATTGC heterozygous End of Table 2.

Based on the chemotype, past research shows that ‘PG 1 19 0125 0002’ mayhave medicinal applications in the treatment of certain cancer types,pain, infection and inflammation, Glaucoma and cardiovascular disease.

THE CLONING PROCESS FOR VEGETATIVE OR ASEXUAL REPRODUCTION

Asexual or vegetative propagation methods (also known as cloning) arewell-known to those skilled in the art. ‘PG 1 19 0125 0002’ is clonedaccording the following method: Coco peat plugs are soaked in pHadjusted water and kept warm. Cuttings measuring 10-12 cm are taken 3nodes from the branch distal meristem and trimmed of lower leaves. Thecuttings are dipped in water and a commercial rooting agent and insertedinto the warmed plugs. Trays are kept in continuous light conditions andhigh humidity for 7 to 15 days until rooted. Rooted plants may betransferred directly into the field for outdoor growth or used forindoor growth. The initial asexual propagation took place in Zeiningen,Switzerland.

It was observed that all of the desired characteristics of each cloneare transferred by vegetative reproduction in a consistent and uniformfashion. The characteristics of ‘PG 1 19 0125 0002’ are stable and thevariety remains true-to-type through multiple generations of vegetativereproduction.

GROWTH OF PLANTS IN INDOOR ENVIRONMENTS

Rooted clones are transferred to two-gallon pots containing growthmedium consisting of a mixture of soil, peat and pearlite in a ratio of3:3:1. Plants are grown in an indoor growth hall under completelysupplied artificial high-pressure sodium (HPS) lighting (E-Papillon,1000W, 400V). The air in the room was circulated with a fan and thehumidity was kept constant at 45-55%. Commercial fertilizer is appliedat a dose of 70-80% of the amount recommended by the manufacturer(Plagron, NL). The plants are grown for 10 days with a light intensitybetween 700-800 μmol m⁻² s-¹, 18 h light/6 h dark day-night photoperiodwith a 24-25° C. day/18° C. night temperature. The photoperiod is thenchanged to a 12 h light/12 h dark day-night cycle and the lightintensity increased by 100 μmol m⁻² s⁻¹ per day for 7 days, at 25° C.day/23° C. night temperatures. During the following 60 days the lightintensity is adjusted to 1400-1500 μmol m⁻² s⁻¹ with a 12 h light/12 hdark day-night photoperiod and a 24-25° C. day/18° C. night temperature.After a total of 84-91 days of growth after cloning, the flowers areharvested.

GROWTH OF PLANTS OUTDOORS

Field growth of ‘PG 1 19 0125 0002’ was performed in Zeiningen,Switzerland during the summer of 2019. The weather during the year of2019 is presented in FIG. 13. Rooted clones were planted in the field onthe 1^(st) of July 2019. Mature flowers were then collected during thefirst two weeks of October.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1: Representative pictures of the ‘PG 1 19 0125 0002’ flowerphenotype when grown under indoor conditions (10 days before harvest).

FIG. 2: ‘PG 1 19 0125 0002’, indoor grown, growth pattern duringvegetative growth phase. A-C and D-F represent individual plantsphotographed from increasing inclination for descriptive purposes. Bothplants were photographed 28-35 days after cloning. Ruler for scale withcm markings.

FIG. 3: ‘PG 1 19 0125 0002’, indoor grown, growth pattern duringflowering. A-C and D-F represent individual plants photographed fromincreasing inclination for descriptive purposes. Both plants werephotographed 1-3 days prior to harvest. Ruler for scale with cmmarkings.

FIG. 4: ‘PG 1 19 0125 0002’ indoor grown growth pattern duringflowering. A and D are a single plant representing the most commonflower structure observed. B and C and individual flowers showing lesscommon but observed flower structure phenotypes. Plants werephotographed 1-3 days prior to harvest.

FIG. 5: ‘PG 1 19 0125 0002’ leaves emerge a very dark green and lightentowards yellow during development. (A) Five leaves throughout indoorgrown development. (B) Leaf 3-5 days after emergence. (C-E) Leavesthrough development but before flower maturation. (F) Senescent leafafter flower maturation. Photos include ruler measures with cm markingsand appropriate Pantone swatch for color comparison.

FIG. 6: ‘PG 1 19 0125 0002’ stem sections from a single plantillustrating a filled/solid stem and not a hollow one common in othervarieties. (Left) Section below the first node on the primary stem,closest to soil level. (Center left) Primary branch. (Center right andright) Flower bearing terminal branches. Ruler for scale with cmmarkings.

FIG. 7: Four representative flowers of indoor grown ‘PG 1 19 0125 0002’illustrating the typical development of the stigma coloration whichbegins a very light green and turn a deep red-brown during maturation.(A-C) Identical flowers photographed with varied Pantone swatches forcolor comparison. Ruler for scale with cm markings.

FIG. 8: Three representative branches of an individual indoor grown ‘PG1 19 0125 0002’ prior to harvest. (A) All three branches photographedtogether. (B-D) The same branches photographed individually. Branchesare photographed with varied Pantone swatches for color comparison andwith a ruler with cm markings for scale.

FIG. 9: A single branch of indoor grown ‘PG 1 19 0125 0002’ showingimmature flowers. (A) The branch with leaves intact showing the ventralred coloration of the petioles. (B) The same branch manually defoliatedaround the apical flowers. (C) A closer image to illustrate the lengthand color of the stigma at this mid flower developmental stage. Branchesare photographed with varied Pantone swatches for color comparison andwith a ruler with cm markings for scale.

FIG. 10: (A) Machine trimmed indoor grown ‘PG 1 19 0125 0002’ flowerharvested at 6.5 weeks after flower initiation. Black bar=5 cm. (B) Adried flower of Santhica 27. Clear differences can be observed in theflower density between the two can be seen. Flower density is acomponent of flower mass and, therefore, cannabinoid production perflower and per plant.

FIG. 11: ‘PG 1 19 0125 0002’, glasshouse grown, growth pattern duringflowering. (A) Side view of a ‘PG 1 19 0125 0002’ flower 10 days priorto harvest. (B-D) An individual plant photographed early in floraldevelopment to show the (B) apical flower, (C) a side view of the wholeplant, (D) a view of the apical inflorescence. Ruler for scale with cmmarkings.

FIG. 12: ‘PG 1 19 0125 0002’ growth pattern during flowering while grownoutdoors near Möhlin, Switzerland. (A) Side view of the apicalinflorescence 14 days prior to harvest. (B) A closer image of the apicalflower. (C) A side view of the apical inflorescence visualizing thedense trichomes on the flowers.

FIGS. 13A-C: FIG. 13A Annual global radiation, FIG. 13B sunshine andFIG. 13C temperatures in Möhlin, the closest weather station to thebreeding site of ‘PG 1 19 0125 0002’. Data range shown is from November2018 to November 2019. Data courtesy of the Swiss Federal Office ofMeteorology and Climatology.

FIG. 14: A graphical representation of the single nucleotidepolymorphisms (SNP) and haplotypes (HAPs) contained within the genome of‘PG 1 19 0125 0002’ when compared to the publicly available referencegenome of cs10 (Assembly: GCF_900626175.1) and shown in Table 2. Thechromosomal reference sequence of cs10 is shown on the left and theapproximate nucleotide position of the cs10 reference sequence is shownbelow. Homo- and heterozygous sequence variants are indicated as blackand red dots, respectively.

DESCRIPTION OF THE NEW VARIETY

‘PG 1 19 0125 0002’ was grown in Zeiningen, Switzerland and observationswere made in the field, in nursery glasshouses as well as in climatecontrolled indoor growth facilities. Observed phenotypes may vary indifferent environmental conditions.

Plants used for the botanical description of the plant are annual,herbaceous, upright, tap-rooted plants. They are Cannabis hybrid speciesand the particular variety described herein is designated the name ‘PG 119 0125 0002’.

Throughout this specification, color names beginning with a small lettersignify that the name of that color, as used in common speech is aptlydescriptive. Color number descriptions were obtained using the PantonePlus Series Color Bridge (ISBN: 978 1-590651-59-9).

‘PG 1 19 0125 0002’ is a cross between multiple pollen donors andseveral pollen acceptors of both hemp-type and high CBD-type Cannabisplants. The initial cross was made during April 2018. The resultant F1seeds were grown, cloned, and the clones used for crossing i.e. selfed.The F2 seed was grown, and from this population ‘PG 1 19 0125 0002’ wasselected. The selection criteria were based on the followingcharacteristics: Maximal CBG content and minimal THC and CBD content inthe mature flower, flower density, and a highly branched plantstructure. The variety was first vegetatively reproduced on the 12^(th)of April 2019 and the resultant plants screened for high total CBGproduction and selected based on production relevant phenotypes. Theprogeny with the most stable desirable chemotype was assigned the name,‘PG 1 19 0125 0002’. ‘PG 1 19 0125 0002’ continues to be vegetativelyreproduced by cloning in Zeiningen, Switzerland.

When ‘PG 1 19 0125 0002’ is compared to the Cannabis variety ‘Santhica27’ (UPOV grant number: 1004490), some similarities and many distinctcharacteristics become apparent. Both ‘PG 1 19 0125 0002’ and ‘Santhica27’ present with CBG as the dominant cannabinoid, with extremely low THCand CBD levels. ‘PG 1 19 0125 0002’, however, displays a up to six timesmore CBG in dried flowers than ‘Santhica 27’. Moreover, the ‘PG 1 190125 0002’ flowers develop into a significantly denser inflorescence.Morphologically, ‘PG 1 19 0125 0002’ is short and highly branched withlarge flowers on each branch, reaching a maximum height of approximately1 meter, whereas ‘Santhica 27’ is tall and mostly unbranched with adominant apical inflorescence, reaching a height of up to 2.5 meters.The characteristics listed above, including branching, flower density,and significantly more CBG per unit mass, results in the ‘PG 1 19 01250002’ variety presenting with a substantially higher harvest index than‘Santhica 27’ with respect to CBG production.

‘PG_1_Pre-19_0125_0004’ is a close relative of ‘PG 1 19 0125 0002’ fromthe Pure Cannabis Research breeding program and displays an almostidentical growth pattern and general phenotype. A clear differencebetween the two varieties can be seen in their cannabinoid profiles (SeeTable 1 above). The cannabinoid profile of the ‘PG_1_Pre-19_0125_0004’flower is clearly CBD-dominant with significant THCA accumulation.

Below is a detailed description of the new variety ‘PG 1 19 0125 0002’(See also Table 3, above). Unless otherwise stated measurements weretaken from plants grown indoors at various stages of development up to11 weeks after cloning.

The ‘PG 1 19 0125 0002’ cultivar is a mixed hybrid of the Cannabis sp.It is naturally obtained and not the result of any genetic modificationtechniques.

-   Plant:    -   -   Plant life form and growth habit.—An annual herbaceous plant            described as broad, upright, tap-rooted.        -   Plant propagation.—Asexually propagated by cutting and            cloning methods.        -   Propagation ease.—‘PG 1 19 0125 0002’ is easy to propagate.        -   Height.—Approximately 50 cm to 100 cm.        -   Width.—Approximately 30 cm to 50 cm.        -   Plant vigor.—Medium — ‘PG 1 19 0125 0002’ bears large            flowers without the requirement for excessive growth.        -   Time to harvest.—From time of cloning the plant will take            approximately 8 weeks to be harvest-ready.        -   Resistance to pathogens.—Partial resistance. It is known to            be moderately susceptible to Botrytis.        -   Genetic modification.—It is naturally obtained and not the            result of any genetic modification techniques.        -   Conditions of flowering.—‘PG 1 19 0125 0002’ flowers once            the daylight period is reduced to 12 hours daylight.        -   Hardiness.—‘PG 1 19 0125 0002’ easily tolerates temperatures            up to 26° C.        -   Breaking action.—This variety has a sturdy main stem with a            “pithy” center. It is not highly flexible, but resistant to            breaking. The flexible side branches are highly resistant to            breaking.        -   Rooting behavior.—When propagated according to PureGene's            standard operating procedures, it roots vigorously at a rate            of 98% within 7-10 days.-   Leaf:    -   -   Arrangement.—Alternating.        -   Shape.—Palmately compound.        -   Structure.—Leaflet blades are very elongated, elliptical,            lanceolate with acute tips and bases.        -   Margins.—Serrated with teeth pointing toward the leaflet            tips.        -   Hairs.—Extremely fine sericreous hairs pointing toward the            leaf tips.        -   Mature leaf measurements.—Leaf length with petiole:            Approximately 23 cm. Petiole length: Approximately 5 cm.            Stipule length and shape: Approximately 0.4 cm to 1.5 cm            linear with acute tip. Leaflet number: About 3 to 9. Middle            leaflet length: width: Approximately 14:3. Teeth on middle            leaflet: Approximately 15 to 30. Width of central leaflets:            Approximately 30 mm to 35 mm for plants grown in indoors            conditions.        -   Leaflet apex shape.—Acuminate acute.        -   Adaxial leaf trichomes (upper and lower surface).—capitate            stalked, capitate sessile and cystolithic trichomes.        -   Abaxial leaf trichomes (upper and lower surface).—Capitate            stalked, capitate sessile and cystolithic trichomes.        -   Abaxial petiole color range.—About Pantone 380-383 UP with            gradual anthocyanin coloration of 7624 U and UP during            flowering.        -   Adaxial petiole color range.—About Pantone 382-385 UP with            gradual anthocyanin coloration of 7624 U and UP during            flowering.        -   Stipule color range.—About Pantone 382-384 UP.        -   Leaf color of the adaxial surface.—About Pantone 347-349 UP,            7734-7745 UP (leaf color changes from very dark green to the            yellow spectrum with age).        -   Leaf color of the abaxial surface.—Pantone 345-347 UP-7730            UP) The lower surface of the leaf changes from a dark green            to light yellow spectrum with age.        -   Leaf glossiness.—Average, becoming more matt at the apical            ends as flowers mature.        -   Midrib shape.—Prominent and continuous throughout each            leaflet.        -   Midrib color.—The midrib is a dark green, similar in shade            to a younger leaf. As the leaf lightens with maturity, the            midrib remains a dark green color (Pantone 349 UP).        -   Aroma.—Earthy with citrus and fruit.-   Stem:    -   -   Shape.—The stem is ribbed with a solid center.        -   Diameter.—Approximately 2 cm to 5 cm.        -   Color.—Varying shades of about light green depending on age            (Pantone 7488 UP).        -   Main stem groove.—Longitudinally ridged with medium grooves            in immature stem, axillary branches, and mature branches            proximal to flowering ends. Grooves gradually smooth out on            older parts of the stem which, at maturity, are smooth and            woody with a solid pith.        -   Stem trichomes.—Capitate sessile glandular trichomes and            unicellular non-glandular trichomes. Only unicellular            non-glandular trichomes on the older, smooth parts of the            stem.        -   Stem internode length.—Short during early vegetative phases            elongating as the plant matures. During late vegetative            phase the average length of the internodes is medium. The            internodes shorten exponentially towards the flowering ends            exhibiting as very short directly below the compound            inflorescence.-   Inflorescence:    -   -   Flowering habit.—During vegetative phase individual flowers            occur at nodes along the stem and branches. Upon flower            initiation flowers appear as clusters, or compound            inflorescences, as a result of higher order branching and            shortened internodes. Terminal inflorescences appear as            compressed and dense while more distal inflorescences            gradually decrease in size and density with a scattered            appearance.        -   Proportion of female flowers.—100%.        -   Inflorescence position.—Above.        -   Flower arrangement.—Individual flowers (bracteoles) on each            mature, compound inflorescence are tightly packed,            congested, concentrated and crowded, touching and            overlapping with stigmas curling over adjacent bracteoles            with maturity.        -   Number of flowers per plant.—About 50 to 100.        -   Individual flower shape.—Each individual flower has one            ovary enclosed in an urceolate bract with two long filiform            stigmas protruding from each ovary and exiting above the            bract.        -   Compound inflorescence shape.—Compound inflorescences are            ovaloid in shape with bilateral symmetry.        -   Flower compound inflorescence diameter.—The average diameter            around the terminal flower compound is 6 cm to 20 cm.        -   Pistil length.—The length from the base of the ovary to the            tips of the stigmas is approximately 2 cm to 10 cm.        -   Style length.—There is no discernible style connecting the            stigmas to the ovary. The stigma refers to the entire length            from the upper curve of the ovary to the tip of the stigmas.        -   Bract shape and color.—Urceolate and about dark green            (Pantone 7731 UP).        -   Stigma shape.—Extremely elongated, elliptical, filiform and            visible as hair-like filaments on the surface of flower            compounds.        -   Stigma length (from the upper surface of the ovary to the            tip of the stigma).—Approximately 2 mm to 10 mm.        -   Stigma color.—The color changes from a about white/light            green (Pantone 7485 UP) to about deep red-brown (Pantone 167            UP) as the flowers mature.        -   Trichome color.—Initially clear becoming cloudy/milky white            and then developing an amber color about Pantone 475 UP at 5            weeks post flowering.        -   Trichome shape.—All flowering parts including the bracteoles            and bracts are covered in capitate stalked, capitate sessile            and bulbous glandular trichomes.        -   Terminal bud shape.—Acute ovoid.        -   Terminal bud color.—At maturity the terminal bud appears            about dark green (Pantone 7731 UP) partially covered by deep            red-brown (Pantone 167 UP) hair-like stigmas.        -   Male flower characteristics.—‘PG 1 19 0125 0002’ clones are            propagated only as females and the male flower            characteristics are not relevant to the physical botanical            characterization of this variety.        -   Cannabinoid contents.—CBG_(max)—9.44%, CBD_(max)—0.21%,            THC_(max)—0.13%.        -   Flower fragrance.—Earthy with citrus notes in line with the            presence of limonene in the terpene profile.        -   Flower shipping quality.—It is a dense appealing flower when            trimmed, cured and packaged according to quality guidelines.            Dry flowers are of high quality and suitable as a            direct-to-consumer commodity.        -   Flower storage life.—A minimum of 1 year if packaged            according to quality guidelines.        -   Flower indoor productivity.—Approximately 0.9 g/watts.        -   Flowering season.—In Zeiningen flowering is initiated around            11 August when the daylength reduces to 14.25 h. This may be            different at different latitudes.-   Seed:    -   -   Shape.—Solitary, ovoid and slightly compressed.        -   Size.—Lateral: Approximately 3 mm. Longitudinal:            Approximately 4 mm.        -   Color of the testa.—Variable but generally a mottled            brown-gray color ranging from About Pantone 4515 U at the            light scale to 7532 UP at the dark scale with very dark            brown about Pantone 7533 UP to black speckling.

What is claimed is:
 1. A new and distinct variety of Cannabis plantnamed ‘PG 1 19 0125 0002’, substantially as illustrated and describedherein.